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SRP0387 Sigma-Aldrich

EZH2 /EED/SUZ12/RbAp48/AEBP2 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥56% (SDS-PAGE)

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Properties

Related Categories Epigenetics, Histone Modifying Enzymes, Methyltransferases, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
biological source   human
recombinant   expressed in baculovirus infected Sf9 cells
assay   ≥56% (SDS-PAGE)
form   aqueous solution
mol wt   87 kDa (EZH2)
packaging   pkg of 20 μg
NCBI accession no.   NM_003797 (EED)
  NM_004456 (EZH2)
  NM_005610 (RbAP48)
  NM_015355 (SUZ12)
  NM_153207 (AEBP2)
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... AEBP2(121536), EED(8726), EZH2(2146), RBBP4(5928), SUZ12(23512)

Description

General description

Mutant version of EZH2 5- member complex with a Tyr-to-Asn mutation on amino acids 646 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), amino acids 2-end (Y646N*) with N-terminal His-tag, MW= 86 kDa, human EED (GenBank Accession No. NM_003797) (amino acids 2-end) with N-terminal Flag-tag, MW= 51 kDa, human SUZ12 (GenBank Accession No. NM_015355) (amino acids 2-end) with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (GenBank Accession No. NM_153207) (amino acids 2-end) with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (GenBank Accession No. NM_005610) (a-a 2- end) with N-terminal His-tag, MW = 48 kDa, co-expressed in Baculovirus expression system. *This mutation is often cited as Y641N in variant 3 GenBank Accession No. NM_001203247).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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