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SRP2056 Sigma-Aldrich

PPARγ, ligand binding domain (165-441) human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym: CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma

  •  NACRES NA.26

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Properties

biological source   human
recombinant   expressed in E. coli
assay   ≥80% (SDS-PAGE)
form   frozen liquid
mol wt   ~33.6 kDa
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   350 μg/mL
color   clear colorless
conjugate   His tagged (N-terminal)
NCBI accession no.   NM_138712
UniProt accession no.   P37231
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PPARG(5468)

Description

Biochem/physiol Actions

There is evidence that a group of closely related nuclear receptors, called peroxisome proliferator-activated receptors (PPARs), may be involved in chronic diseases such as diabetes, obesity, artherosclerosis and cancer. The PPARs were first cloned as the nuclear receptors that mediate the effects of synthetic compounds called peroxisome proliferators on gene transcription. It soon became clear that eicosanoids and fatty acids can also regulate gene transcription through PPARs. They bind a specific element in the promoter region of target genes only as a heterodimer with the receptor for 9- cis retinoic acid, RXR (retinoid X receptor). Binding of the ligand of either receptor can activate the complex, but binding of both ligands simultaneously is more potent. Three PPAR isotypes have been identified: α, β (also called NUC1) and γ. PPARα is expressed most in brown adipose tissue and liver, then kidney, heart and skeletal muscle. PPARγ is mainly expressed in adipose tissue, and to a lesser extent in colon, the immune system and the retina. PPARβ is found in many tissues but the highest expression is in the gut, kidney and heart. PPARγ influences the storage of fatty acids in the adipose tissue. With the C/EBP transcription factors, PPARγ is part of the adipocyte differentiation program that induces the maturation of pre-adipocytes into fat cells. Most of the PPARγ target genes in adipose tissue are directly implicated in lipogenic pathways, including lipoprotein lipase (LPL), adipocyte fatty acid binding protein (A-FABP or AP2), acyl-CoA synthase and fatty acid transport protein (FATP). In addition, PPARγ is a direct target gene of the transcription factor sterol response element binding protein 1 (SREBP1) emphasizing the cooperative and additive functions between these two types of receptor.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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