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SRP2072 Sigma-Aldrich

HDAC3 human

recombinant, expressed in insect cells, ≥70% (SDS-PAGE)

Synonym: HD3, RPD3-2, RPD3

  •  NACRES NA.26

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones, HDACs,
biological source   human
recombinant   expressed in insect cells
assay   ≥70% (SDS-PAGE)
form   frozen liquid
mol wt   ~50 kDa
packaging   pkg of 5 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   300 μg/mL
color   colorless to clear
NCBI accession no.   NM_003883
UniProt accession no.   O15379
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... HDAC3(8841)

Description

Biochem/physiol Actions

The nucleosomal histones can be modified through reversible acetylation by histone acetyltransferases (HATs) and deacetylases (HDACs). Acetylation and deacetylation of histone proteins alters chromosome structure and affects transcription factor access to DNA. HDAC3 has an open reading frame of 428 amino acids. HDAC3 shares 53 % amino acid identity with HDAC1 and 52 % with HDAC2. Observations indicate that class II HDACs regulate transcription by bridging the enzymatically active NCOR2-HDAC3 complex and select transcription factors. All HDACs were found to be ubiquitously expressed in immune and non-immune tissues. In human myeloid leukemia THP-1 cells, HDAC3 transfection resulted in increased size, aberrant nuclear morphology and cell cycle G2/M cell accumulation. Functional activity of the expressed HDAC3 protein was confirmed in alpha-HDAC3 antibody immunoprecipitates by a histone deacetylase assay. Study suggests the participation of HDACs in cell cycle progression and activation.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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