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SRP2091 Sigma-Aldrich

FGF-1 human

recombinant, expressed in insect cells, ≥85% (SDS-PAGE)

Synonym: AFGF, ECGF-beta, ECGF, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, FGF-1 (FGF acidic), Fibroblast Growth Factors (FGF),
biological source   human
recombinant   expressed in insect cells
assay   ≥85% (SDS-PAGE)
form   frozen liquid
mol wt   ~19.2 kDa
packaging   pkg of 5 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   500 μg/mL
color   colorless to clear
NCBI accession no.   NM_000800
UniProt accession no.   P05230
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... FGF1(2246)

Description

General description

The gene FGF1 (fibroblast growth factor 1) encodes a member of the fibroblast growth factor (FGF) family. It encodes a pro-angiogenic protein that is ubiquitously expressed and is secreted via transmembrane translocation from the cells through a nonclassical secretory pathway in response to stress. It has the ability to permeate membranes composed of acidic phospholipids.

Application

FGF-1 (fibroblast growth factor 1) has been used as a growth factor to study the effect of FGF1 on early branching events in isolated endoderm obtained from embryonic mouse lung. It has also been used to study the neuroprotection effect of FGF1 on cerebellar granule neurons obtained from rats.

Biochem/physiol Actions

Acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2) are ubiquitous cytokines found in many tissues. They have effects on multiple cell types derived from mesoderm and neuroectoderm, including endothelial cells. FGF proteins are small peptides of 155 to 268 amino acid residues. The degree of sequence identity between different family members is 30-60 % in a "central domain" of approx. 120 amino acids. This domain confers to FGFs a common tertiary structure and the ability to bind to heparin. Secreted FGFs signal to target cells by binding and activating cell-surface tyrosine kinase FGF receptors (FGFRs; 6, 7). The function of FGFs and FGFRs during embryonic development and adult physiology has been addressed by gain- and loss-of-function experiments in several animal model organisms. These studies have shown that FGFs act as key regulators of developmental events.

The protein fibroblast growth factor 1, functions intracellularly to protect cells against stress. The protein functions as an angiogenic factor that participates in tissue repair, carcinogenesis, and maintenance of vasculature stability. Its expression is found to be upregulated in in patients with rheumatic arthritis. It serves as a prognostic biomarker in several cancers and a potential therapeutic target for patients with non-small cell lung cancer.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
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References

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