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SRP2158 Sigma-Aldrich

Pregnane X Receptor human

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

Synonym: BXR, ONR1, PAR1, PAR2, PAR, PARq, PRR, PXR

  •  NACRES NA.26

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Gene Regulation and Expression, Transcription Factors
biological source   human
recombinant   expressed in insect cells
assay   ≥80% (SDS-PAGE)
form   frozen liquid
mol wt   ~51.6 kDa
packaging   pkg of 5 μg
storage condition   avoid repeated freeze/thaw cycles
concentration   650 μg/mL
color   clear colorless
NCBI accession no.   NM_003889
UniProt accession no.   O75469
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... NR1I2(8856)

Description

Biochem/physiol Actions

The nuclear pregnane X receptor (PXR; NR1I2) is an important component of the body′s adaptive defense mechanism against toxic substances including foreign chemicals (xenobiotics). PXR is activated by a large number of endogenous and exogenous chemicals including steroids, antibiotics, antimycotics, bile acids, and the herbal antidepressant St. John′s wort. PXR is known to regulate the expression of several additional genes encoding proteins involved in xenobiotic metabolism, including multidrug resistance protein 1 (MDR1), multidrug resistance-associated protein 2 (MRP2), and organic anion transporter polypeptide 2. Elucidation of the three-dimensional structure of the PXR ligand binding domain revealed that it has a large, spherical ligand binding cavity that allows it to interact with a wide range of hydrophobic chemicals. Thus, unlike other nuclear receptors that interact selectively with their physiological ligands, PXR serves as a generalized sensor of hydrophobic toxins. PXR binds as a heterodimer with the 9-cis retinoic acid receptor (NR2B) to DNA response elements in the regulatory regions of cytochrome P450 3A monooxygenase genes and a number of other genes involved in the metabolism and elimination of xenobiotics from the body. Although PXR evolved to protect the body, its activation by a variety of prescription drugs represents the molecular basis for an important class of harmful drug-drug interactions. Thus, assays that detect PXR activity will be useful in developing safer prescription drugs.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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