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SRP3003 Sigma-Aldrich

Activin A human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC), cell culture tested

Synonym: Activin beta-A, FRP, Inhibin beta-1

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Properties

Related Categories Cell Biology, Cell Culture, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation, Cytokines, Growth Factors, and Chemokines for Stem Cell Expansion, Embryonic Stem Cell Biology Growth Factors,
biological source   human
recombinant   expressed in E. coli
assay   ≥97% (HPLC)
  ≥97% (SDS-PAGE)
form   lyophilized
potency   ≤2.0 ng/mL ED50
mol wt   mol wt 26.0 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
suitability   cell culture tested
UniProt accession no.   P08476
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... INHBA(3624)
mouse ... Inhba(16323)
rat ... Inhba(29200)

Description

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized with no additives.

Application

Activin A has been used in monolayer induction for endoderm (hepatocyte) differentiation.

Activin A human has been used to study rRNA gene transcription during directed differentiation of human embryonic stem cells.

Biochem/physiol Actions

Activin A (inhibin βA) is a TGF (transforming growth factor)-β family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively. Activin A plays a central role in reproduction by regulating the levels of follicle stimulating hormone (FSH). It is involved in hematopoiesis, embryogenesis and osteogenesis. It exhibits both tumor suppressing and promoting effects. In underweight and overweight humans, high levels of Activin A in serum might be associated with the pathogenesis of asthma. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.

General description

Activins (also referred to as inhibins) are generally dimers of three subunits: inhibin βA, inhibin βB, and inhibin α. Recombinant human Activin A (inhibin βA) is a 26kDa disulfide-linked homodimer of two bA chains, each containing 116 amino acid residues. The sequence of mature Activin A is completely homologous between human, mouse, and rat species.

Sequence

GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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