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SRP3013 Sigma-Aldrich

Beta D-4 human

recombinant, expressed in E. coli, ≥98% (HPLC), cell culture tested

Synonym: Beta-defensin 104, DEFB104A

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Properties

Related Categories A - K, Antibacterial, Antibiotic Peptides, Antibiotics, Antibiotics A to Z,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
form   lyophilized
mol wt   6.0 kDa
packaging   pkg of 20 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
Mode of action   cell membrane | interferes
UniProt accession no.   Q8WTQ1
antibiotic activity spectrum   fungi
  mycobacteria
  viruses
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... DEFB104A(140596)

Description

General description

Chemical structure: peptide

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four human b-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. b-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney. In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes. Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues.

Biochem/physiol Actions

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues.

Sequence

EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP

Physical form

Lyophilized from 10 mM Acetic Acid.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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