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SRP3028 Sigma-Aldrich

EGF-receptor human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC), cell culture tested

Synonym: ErbB1

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Properties

Related Categories Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Culture, Epidermal Growth Factors (EGF),
biological source   human
recombinant   expressed in CHO cells
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
mol wt   97.5 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   P00533
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... EGFR(1956)

Description

General description

Epidermal growth factor receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. EGFR can be activated by binding EGF or at least six other structurally related protein ligands, including transforming growth factor α (TGFα), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), amphiregulin, epiregulin, and epigen. The gene encoding it is localized on human chromosome 7p11.2. Recombinant soluble human EGFR is a 621 amino acid glycoprotein comprising the extracellular domain of EGFR, and migrates at an apparent MW of 97.5 kDa by SDS-PAGE analysis under reducing conditions.

Biochem/physiol Actions

Upon activation by ligands, epidermal growth factor receptor (EGFR, ErbB1) initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers.

Sequence

LEEKKVCQGT SNKLTQLGTF EDHFLSLQRM FNNCEVVLGN LEITYVQRNY DLSFLKTIQE VAGYVLIALN TVERIPLENL QIIRGNMYYE NSYALAVLSN YDANKTGLKE LPMRNLQEIL HGAVRFSNNP ALCNVESIQW RDIVSSDFLS NMSMDFQNHL GSCQKCDPSC PNGSCWGAGE ENCQKLTKII CAQQCSGRCR GKSPSDCCHN QCAAGCTGPR ESDCLVCRKF RDEATCKDTC PPLMLYNPTT YQMDVNPEGK YSFGATCVKK CPRNYVVTDH GSCVRACGAD SYEMEEDGVR KCKKCEGPCR KVCNGIGIGE FKDSLSINAT NIKHFKNCTS ISGDLHILPV AFRGDSFTHT PPLDPQELDI LKTVKEITGF LLIQAWPENR TDLHAFENLE IIRGRTKQHG QFSLAVVSLN ITSLGLRSLK EISDGDVIIS GNKNLCYANT INWKKLFGTS GQKTKIISNR GENSCKATGQ VCHALCSPEG CWGPEPRDCV SCRNVSRGRE CVDKCNLLEG EPREFVENSE CIQCHPECLP QAMNITCTGR GPDNCIQCAH YIDGPHCVKT CPAGVMGENN TLVWKYADAG HVCHLCHPNC TYGCTGPGLE GCPTNGPKIP S

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

EGF/EGFR signaling pathway

Background Epidermal Growth Factor Receptors (EGFR) and ligands     Receptor activation EGFR Signaling     Ras/Raf signaling cascade     Phosphatidylinositol 3-kinase/Akt signaling cascade     Signal...
Keywords: Apoptosis, Cancer, Cell proliferation, Degradations, Diseases, Electron spin resonance, Gene expression, Genetic, Glycosylations, Growth factors, Ligands, Transcription, Transduction

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Peer-Reviewed Papers
15

References

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