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SRP3033 Sigma-Aldrich

Epiregulin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: EREG

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Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Core Bioreagents,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   <0.2 ng/mL
mol wt   5.6 kDa
packaging   pkg of 25 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   O14944
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... EREG(2069)

Description

General description

EREG (epiregulin) is mainly known as a ligand of EGFR (epidermal growth factor receptor) and ErbB-4 (receptor tyrosine-protein kinase). It induces tyrosine phosphorylation of EGFR, ErbB-2, ErbB-3 and ErbB-4. The mechanism of action involves interaction with EGFR, dimerization of EGFR and induction of autophosphorylation. The EREG gene is mapped to human chromosome 4q13.
Recombinant human Epiregulin is a 5.6kDa monomeric protein, containing 50 amino residues, which corresponds to the mature secreted Epiregulin sequence.

Application

Epiregulin human has been added in the culture medium to study the effect of epiregulin on epithelial invasion.

Biochem/physiol Actions

EREG (epiregulin)-mediated EGFR (epidermal growth factor receptor) phosphorylation is responsible for the activation of various pathways, including the mitogen-activated protein kinase (MAPK), phosphoinositide 3-kinase (PI3K) and STAT5 (signal transducer and activator of transcription 5) responses. These pathways are involved in proliferation, cell survival, and angiogenesis. EREG is upregulated in colon, breast and ovarian cancers.

Sequence

MVAQVSITKC SSDMNGYCLH GQCIYLVDMS QNYCRCEVGY TGVRCEHFFL

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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