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SRP3043 Sigma-Aldrich

FGF-basic human

Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), cell culture tested

Synonym: Catabolin, Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Lymphocyte-activating factor (LAF), Mononuclear Cell Factor (MCF)

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Description

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from 5 mM Tris, pH 7.6 + 150mM NaCl.

Application

FGF-basic has been used to supplement serum-free DMEM during culture of HeLa cells.

Biochem/physiol Actions

FGF-basic (fibroblast growth factor), also referred to as FGF2, activates the ERK (extracellular signal-regulated kinase) cascade and facilitates self-renewal of hESCs (human embryonic stem cells). FGF2 positively regulates cell survival and/or proliferation. It is found to negatively regulate BMP (bone morphogenetic protein) signaling, a pathway that induces non-neuronal differentiation.

General description

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.

Sequence

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

Fibroblast Growth Factor Family (FGF)

Fibroblast Growth Factors (FGFs) are potent regulators of cell proliferation, differentiation and function and are critically important in normal development, tissue maintenance, wound repair and ang...
Keywords: Angiogenesis, Cancer, Cardiovascular, Cell proliferation, Diabetes, Diseases, Gene expression, Growth factors, Ligands

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Peer-Reviewed Papers
15

References

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