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SRP3071 Sigma-Aldrich

IL-10 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: B-TCGF, CSIF, TGIF

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Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤2.0 ng/mL ED50
mol wt   18.6 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   P22301
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... IL10(3586)

Description

General description

IL-10 (interleukin 10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. The gene is mapped to human chromosome 1q31-1q32. Recombinant human IL-10 is an 18.6kDa protein of 161 amino acid residues. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells.

Application

IL-10 human has been used to study the effect of IL-10 on endothelial nitric oxide synthase and nitric oxide in African American human umbilical vein endothelial cells. It has also been used to study the effect of IL-10 on leukotriene (LT) A4 hydrolase and LTC4 synthase protein expression in and secretion from, an inflamed porcine endometrium.

Biochem/physiol Actions

IL-10 (interleukin 10) is an anti-inflammatory cytokine. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF (tumor necrosis factor)-α. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Polymorphisms in the IL-10 gene are associated with higher risk of preeclampsia, ankylosing spondylitis, nasopharyngeal carcinoma and basal cell carcinoma. Polymorphism in IL-10 at -1082A/G is associated with diabetic nephropathy.

Sequence

MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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