EMAIL THIS PAGE TO A FRIEND
SRP3074 Sigma-Aldrich

IL-12p40 human

recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Purchase

Description

General description

IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23. Recombinant human IL-12 p40 is a 40.0 kDa protein containing 306 amino acid residues.

The interleukin-12 subunit β (IL12β) gene is localized on human chromosome 5q31–33. The encoded protein is part of IL-12, which is a heterodimer. It is mainly expressed by antigen-presenting cells (APCs).

Biochem/physiol Actions

IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Recombinant human IL-12 p40 is a 40.0 kDa protein containing 306 amino acid residues.

Interleukin-12 subunit β (IL12β) is an immunoregulatory cytokine. It is involved in pathogenic defense responses and is also suggested as an anticancer therapeutic agent. IL12B is responsible for polarizing naive CD4+ cells toward a TH1 (T helper) phenotype and inducing IFN-γ (interferon) secretion by T and natural killer (NK) cells. This protein also stimulates the differentiation of cytotoxic CD8+ cells and enhances the activity of NK cells. IL12B is linked with many autoimmune diseases, such as psoriasis, systemic lupus erythematosus, Crohn’s disease and ulcerative colitis.

Sequence

p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Physical form

Lyophilized from 1x PBS, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?