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SRP3090 Sigma-Aldrich

IL-3 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: HCGF, MCGF (Mast cell growth factor), Multi-CSF, P-cell stimulation factor

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤0.1 ng/mL ED50
mol wt   mol wt 15.0 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   P08700
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... IL3(3562)

Description

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized with no additives.

Biochem/physiol Actions

IL3 (interleukin 3) promotes the survival, proliferation and differentiation of haematopoietic progenitor cells. It also functions as an activator of basophils thus, resulting in significant increase in antigen and IgE-mediated release of cytokines and other mediators. Certain variants in this gene confer protection against malaria attack, and thus, this gene might play a role in the pathophysiology of falciparum malaria. SNP (single nucleotide polymorphism) rs40401 in this gene and smoking have a positive correlation with asthma in Japanese women. IL3 is involved in brain development and has a direct influence on the proliferation and survival of neural progenitor cell population, thereby also controlling human brain volume.

General description

IL3 (interleukin 3) is a haematopoietic growth factor, the gene for which is localized to human chromosome locus 5.
Recombinant human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.

Sequence

APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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