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SRP3093 Sigma-Aldrich

IL-4 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: B cell stimulating factor (BSF-1), BCDF, BCGF

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Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines & Growth Factors,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤0.2 ng/mL ED50
mol wt   14.9 kDa
packaging   pkg of 20 μg
impurities   <0.1 EU/μg endotoxin, tested
color   off-white to yellow
suitability   cell culture tested
UniProt accession no.   P05112
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... IL4(3565)

Description

General description

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.

Application

Interleukin-4 (IL-4) has been used as a stimulant of M2 state of microglia that is associated with wound healing and debris clearance.

Biochem/physiol Actions

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues.

Sequence

MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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