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SRP3096 Sigma-Aldrich

IL-6 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym: 26 kDa protein, BSF-2, HPGF, HSF, IFN-b2, MGI-2

  •  NACRES NA.32

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Properties

Related Categories CNTF Family - includes ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF) and interleukin-6 (IL-6), Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤0.1 ng/mL ED50
mol wt   20.9 kDa
packaging   pkg of 20 μg
application(s)   cell culture | mammalian: suitable
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
UniProt accession no.   P05231
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... IL6(3569)

Description

General description

IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kDa protein containing 184 amino acid residues.

Application

IL-6 human has been used for STAT3 (signal transducer and activator of transcription 3) activation. It has also been used as a standard for detection of IL-6 in Ben-Men-I cells (human WHO grade I meningioma derived, hTERT immortalized cells).

Biochem/physiol Actions

IL-6 (interleukin 6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease, and plasmacytoma/multiple myeloma.

Sequence

PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Protocol Guide: MTT Assay for Cell Viability and Proliferation

The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. This colorimetric assay is based on the reduction of a yellow tetrazoli...
Keywords: Antibiotics, Cancer, Cell proliferation, Enzyme-linked immunosorbent assay, Growth factors, Indicators, Metabolism, Reductions

Protocol Guide: XTT Assay for Cell Viability and Proliferation

The non-radioactive, colorimetric assay system using XTT (sodium 3´-[1- (phenylaminocarbonyl)- 3,4- tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) was first described by Scudiero,...
Keywords: Antibiotics, Cell proliferation, Enzyme-linked immunosorbent assay, Growth factors, Indicators, Metabolism, Reductions

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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