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SRP3098 Sigma-Aldrich

IL-8 (77aa) (CXCL8) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: CXCL8, NAP-1, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF)

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Chemokines, Cytokines and Growth Factors,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
mol wt   8.9 kDa
packaging   pkg of 25 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   P10145
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... IL8(3576)

Description

General description

Interleukin-8 (IL-8) is a proinflammatory CXC chemokine that can signal through the C-X-C motif chemokine receptors 1 and 2 (CXCR1 and 2). It is also called as C-X-C motif chemokine ligand 8 (CXCL8). IL-8 is secreted by monocytes and endothelial cells. The gene encoding IL-8 is localized on human chromosome 4q13.3. Recombinant human IL-8 (endothelial-derived) is an 8.9kDa protein containing 77 amino acid residues.

Application

IL-8 (77aa) (CXCL8) human has been used as a G protein-coupled receptor (GPCR) agonist.

Biochem/physiol Actions

Interleukin-8 (IL-8) chemoattracts and activates neutrophils. It is involved in guiding neutrophils and oligodendrocytes to sites of infection. The protein also stimulates angiogenesis and tumor growth. It is also associated with inflammatory diseases.

Sequence

AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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