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SRP3126 Sigma-Aldrich

NP-1 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym: Cryptdin, HNP-1, Neutrophil Peptide-1, alpha Defensin-1

  •  NACRES NA.32

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Properties

Related Categories Antibacterial, Antibiotic Peptides, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
mol wt   3.4 kDa
packaging   pkg of 20 μg
application(s)   cell culture | mammalian: suitable
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
Mode of action   cell membrane | interferes
UniProt accession no.   O14786
antibiotic activity spectrum   fungi
  mycobacteria
  viruses
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... NRP1(8829)

Description

General description

Chemical structure: peptide

Defensins (α and β) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins which include neutrophil peptides-1, -2 and -3, (NP-1, NP-2 and NP-3) are distinguished from the β-defensins by the pairing of their three disulfide bonds. Neutrophil-1 (NP-1) is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. The protein contains a six-cysteine motif that forms three intra-molecular disulfide bonds. It is a transmembrane glycoprotein. Recombinant human NP-1 is a 3.4kDa protein containing 30 amino acid residues.

Biochem/physiol Actions

Neutrophil peptide-1 (NP-1) exhibits antimicrobial activity. It also has a chemotactic function in dendritic cells. It has a role in the growth and metastasis of cancer cells. The protein acts as multifunctional co-receptor and interacts with various signaling cascades. NP-1 associates with epithelial growth factor (EGF) and its receptor (EGFR). It also interacts with hepatocyte growth factor (HGF) and its receptor c-Met.

Sequence

ACYCRIPACI AGERRYGYCI YQGRLWAFCC

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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