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SRP3140 Sigma-Aldrich

PECAM-I human

recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE), ≥97% (HPLC), cell culture tested

Synonym: CD31 antigen, EndoCAM, Platelet endothelial cell adhesion molecule

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Properties

Related Categories Adhesion Molecules and Related Proteins, Attachments Factors, Cell Biology, Cell Signaling and Neuroscience, Core Bioreagents,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥97% (HPLC)
  ≥97% (SDS-PAGE)
form   lyophilized
potency   1.0-1.5 μg/mL ED50
mol wt   mol wt 80.0-95.0 kDa
packaging   pkg of 50 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   P16284
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... PECAM1(5175)

Description

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Biochem/physiol Actions

PECAM1 (platelet and endothelial cell adhesion molecule 1) forms an essential part of the endothelial cell intercellular junctions in vascular beds. Its extracellular domain is involved in homo- and heterophilic interactions, and its cytosolic domain is involved in the transduction of a variety of cellular signaling. It controls vascular inflammatory response as it functions as both pro-inflammatory and anti-inflammatory molecule. Its capacity for hemophilic interactions renders it essential for leukocyte transmigration, interendothelial cell bindings, and vascular permeability regulation. This molecule is thought to be involved in the pathogenesis of various disorders such as thrombosis, cardiovascular disease, inflammation, and cancer.

General description

PECAM1 (platelet and endothelial cell adhesion molecule 1), a member of the Ig (immunoglobulin) superfamily, is a multifunctional vascular cell adhesion and signaling molecule. It is a surface molecule found on circulating leukocytes, endothelial cells, and platelets. It is a glycoprotein with a molecular weight of 130kDa, and is composed of a single-chain of six Ig-like extracellular homology domains (total 574-residues), a transmembrane portion of 19 residues, and a cytoplasmic tail containing 118 amino acids. This gene is localized to human chromosome 17q23, and is a 75kb gene composed of 16 exons.
Monomeric glycosylated PECAM-1 migrates at an apparent molecular weight of approximately 80.0-95.0kDa by SDS-PAGE analysis under reducing conditions.

Sequence

ENSFTINSVD MKSLPDWTVQ NGKNLTLQCF ADVSTTSHVK PQHQMLFYKD DVLFYNISSM KSTESYFIPE VRIYDSGTYK CTVIVNNKEK TTAEYQLLVE GVPSPRVTLD KKEAIQGGIV RVNCSVPEEK APIHFTIEKL ELNEKMVKLK REKNSRDQNF VILEFPVEEQ DRVLSFRCQA RIISGIHMQT SESTKSELVT VTESFSTPKF HISPTGMIME GAQLHIKCTI QVTHLAQEFP EIIIQKDKAI VAHNRHGNKA VYSVMAMVEH SGNYTCKVES SRISKVSSIV VNITELFSKP ELESSFTHLD QGERLNLSCS IPGAPPANFT IQKEDTIVSQ TQDFTKIASK SDSGTYICTA GIDKVVKKSN TVQIVVCEML SQPRISYDAQ FEVIKGQTIE VRCESISGTL PISYQLLKTS KVLENSTKNS NDPAVFKDNP TEDVEYQCVA DNCHSHAKML SEVLRVKVIA PVDEVQISIL SSKVVESGED IVLQCAVNEG SGPITYKFYR EKEGKPFYQM TSNATQAFWT KQKASKEQEG EYYCTAFNRA NHASSVPRSK ILTVRVILAP WK

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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