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SRP3171 Sigma-Aldrich

TGF-beta 3-E. coli human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: Transforming Growth Factor-beta3

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Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines & Growth Factors,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤0.05 ng/mL ED50
mol wt   25.0 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white
suitability   cell culture tested
UniProt accession no.   P10600
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... TGFB3(7043)

Description

General description

The gene TGF-β3 (transforming growth factor β 3) is mapped to human chromosome 14q24.3. The three mammalian isoforms of TGF-β, TGF-β1, β2, β3 elicit similar biological responses. TGF-β proteins are made as precursor molecules which are cut to generate a mature carboxy-terminal region of 110-140 amino acids. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. Recombinant TGF-β3 is a 25.0kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Application

TGF-β3 (transforming growth factor β 3) human has been used in the media for the chondrogenic differentiation of the stromal cells. It has been used in the culture medium to allow attachment of primary human dermal fibroblasts to the scaffolds.

Biochem/physiol Actions

The three mammalian isoforms of TGF-β (transforming growth factor β), TGF-β1, β2, β3, are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. TGF-β3 is the predominant TGF-β in the testis and controls the blood-testis barrier activity. Polymorphisms in this gene might be associated with male infertility. Heterozygous mutation in the gene at position Arg300 might be associated with increased TGF-β signaling, leading to a disorder with phenotypic overlap with Marfan syndrome and Loeys-Dietz syndrome. TGF-β3 is involved in the mesenchymal stem cells (MSCs)-mediated inhibition of fibroblast proliferation and decreases skin fibrosis.

Sequence

ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

Physical form

Lyophilized from 0.1% TFA

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 °C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 °C to -80 °C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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