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SRP3178 Sigma-Aldrich

TPO human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand

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Properties

Related Categories Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤1.0 ng/mL ED50
mol wt   mol wt 18.6 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white
suitability   cell culture tested
UniProt accession no.   P40225
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... THPO(7066)

Description

Biochem/physiol Actions

Thrombopoietin (TPO) stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the cellular proto-oncogene (c-mpl) receptor and acts as an important regulator of circulating platelets. High levels of TPO have been observed in patients with aplastic anemia. Human and murine TPO exhibits cross-species reactivity.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from 0.1% TFA.

General description

Thrombopoietin (TPO) is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It is an acidic glycoprotein. The gene encoding it is localized on human chromosome 3q27.1. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6kDa), which contains the erythropoietin-like domain of the full length TPO protein.

Sequence

SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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