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SRP3181 Sigma-Aldrich

VCAM-I human

recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: CD106, INCAM-100, MGC108734, MGC99561, VCAM

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Properties

Related Categories Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Signaling and Neuroscience, Cytoskeleton and Extracellular Matrix,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   0.8-1.0 μg/mL ED50
mol wt   74.1 kDa
packaging   pkg of 50 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white
suitability   cell culture tested
UniProt accession no.   P19320
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... VCAM1(7412)

Description

General description

Vascular cell adhesion molecule 1 (VCAM-1) is a 110kDa cell surface integral membrane glycoprotein that belongs to the Ig (immunoglobulin)-related superfamily of adhesion molecules. The human VCAM-1 gene codes for a 715 amino acid transmembrane glycoprotein containing a 19 amino acid cytoplasmic domain, a 22 amino acid transmembrane domain, and a 674 amino acid extracellular domain. It is expressed on endothelial cells. Recombinant human VCAM-1 is a 110kDa glycoprotein comprising the extracellular domain (673 amino acid residues) of VCAM-1. Monomeric glycosylated VCAM-1 migrates at an apparent molecular weight of approximately 74.1kDa by SDS-PAGE analysis under reducing conditions.

Biochem/physiol Actions

The primary function of VCAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 may play a vital role in the development of several diseases, including atherosclerosis and rheumatoid arthritis. The protein has a crucial role in early embryonic development. It also has a role in lymphocyte trafficking and homeostasis of lymphocyte development.

Sequence

FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYS FPKDPEIHLS GPLEAGKPIT VKCSVADVYP FDRLEIDLLK GDHLMKSQEF LEDADRKSLE TKSLEVTFTP VIEDIGKVLV CRAKLHIDEM DSVPTVRQAV KELQVYISPK NTVISVNPST KLQEGGSVTM TCSSEGLPAP EIFWSKKLDN GNLQHLSGNA TLTLIAMRME DSGIYVCEGV NLIGKNRKEV ELIVQEKPFT VEISPGPRIA AQIGDSVMLT CSVMGCESPS FSWRTQIDSP LSGKVRSEGT NSTLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFP RDPEIEMSGG LVNGSSVTVS CKVPSVYPLD RLEIELLKGE TILENIEFLE DTDMKSLENK SLEMTFIPTI EDTGKALVCQ AKLHIDDMEF EPKQRQSTQT LYVNVAPRDT TVLVSPSSIL EEGSSVNMTC LSQGFPAPKI LWSRQLPNGE LQPLSENATL TLISTKMEDS GVYLCEGINQ AGRSRKEVEL IIQVTPKDIK LTAFPSESVK EGDTVIISCT CGNVPETWII LKKKAETGDT VLKSIDGAYT IRKAQLKDAG VYECESKNKV GSQLRSLTLD VQGRENNKDY FSP

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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