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SRP3186 Sigma-Aldrich

Vitronectin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym: Epiboin, S-Protein, Serum-spreading factor, V75, VTN

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Properties

Related Categories Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Angiogenesis Regulators, Application Index, Attachment Factors, Attachments Factors,
Quality Level   200
biological source   human
recombinant   expressed in HEK 293 cells
sterility   sterile
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
potency   ≤0.5 μg/mL EC50
mol wt   75 kDa
packaging   pkg of 250 μg
application(s)   cell culture | mammalian: suitable
impurities   <0.1 EU/μg endotoxin, tested
color   white
UniProt accession no.   P04004
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... VTN(7448)

Description

General description

Vitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers. Recombinant human Vitronectin is a 459 amino acid single chain monomeric protein, which migrates at an apparent molecular weight of 75kDa by SDS-PAGE under reducing conditions. Recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media.

Application

Vitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates.

Biochem/physiol Actions

The multimeric Vitronectin can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin αvβ3 enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. Vitronectin is involved in wound repair and also controls complement system. It works as a marker of profibrotic activity in liver, heart and kidney.

Sequence

DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Physical form

Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Protocols

Coating Tissue Culture Plates with Vitronectin

Vitronectin is used to coat culture vessels for cells attachment. Below is a suggested coating protocol for a 96-well plate, at a coating concentrations of 0.5 µg/cm2. Note that the optimal concentra...
Keywords: Cell attachment, PAGE

Serum free cancer cell line media

Historically, cancer cell lines have been cultured almost exclusively in culture media supplemented with significant amounts of fetal bovine serum (FBS). Like other undefined media supplements, fetal...
Keywords: Adhesion, Cancer, Cell culture, Culture media, Endocrinology, Genetic, Genomics, Hormones, Inflammation

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
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Peer-Reviewed Papers
15

References

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