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SRP3197 Sigma-Aldrich

FGF-acidic from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), cell culture tested

Synonym: ECGF-beta, FGF-1, Fibroblast Growth Factor-acidic, HBGF-1

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Description

General description

FGF1 (fibroblast growth factor 1) belongs to the FGF family of genes. In humans, FGF1 is alternatively spliced into four forms: FGF1A (in kidney), FGF1B (in brain), FGF1-C and -D (in vascular smooth muscle cells and fibroblasts). FGF1 interacts with tyrosine kinase FGF receptors (FGFR1-4) with high affinity. It can translocate between the cytosol and nucleus.
Recombinant murine FGF-acidic is a 15.9kDa protein consisting of 141 amino acid residues.

Application

FGF-acidic from mouse has been used to induce the expression of PTPIP51 (protein tyrosine phosphatase interacting protein 51) in mouse hippocampal cells.

Biochem/physiol Actions

FGF1 (fibroblast growth factor 1) is mainly involved in cell growth, proliferation and neurogenesis. In high-fat diet (HFD) mice, FGF1 is upreglated in adipose tissue. Absence of FGF1 in mice results in aggressive diabetic phenotype as well as adipose expansion in the presence of HFD. The PPARγ (peroxisome proliferator-activated receptor γ)-FGF1 axis helps in regulating metabolic homeostasis and insulin sensitization in mice. FGF1 also participates in wound healing, post-ischemic heart repair and making of collaterals after hindlimb ischemia.

Sequence

MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 7.2 + 100 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 5mM Sodium Phosphate, pH 7.0-7.2 to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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