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SRP3198 Sigma-Aldrich

flt3-Ligand from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: Fms-related tyrosine kinase 3 ligand

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Properties

Related Categories Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Culture, General Stem Cell Biology,
biological source   mouse
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   5.0-8.0 ng/mL
mol wt   18.6 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   P49772
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... FLT3LG(14256)

Description

General description

Flt3l (FMS-like tyrosine kinase 3 ligand) is a cytokine and a hematopoietic growth factor. It acts as a ligand for FLT3 receptor. Alternative splicing of FLT3L results in both membrane-bound and soluble forms. Soluble form is a result of proteolytic cleavage of the membrane isoform. It has a wide range of tissue expression.
Recombinant murine Flt3-Ligand is a soluble 18.6 kDa protein consisting of 163 amino acid residues.

Biochem/physiol Actions

Flt3l (FMS-like tyrosine kinase 3 ligand) acts as a stimulant of hematopoietic progenitor cell proliferation. It facilitates the commitment of lymphoid lineage, and aids in dendritic cell (DC) development. It is involved in myoblast differentiation, and drives skeletal muscle regeneration. It acts on p120RasGAP (rat sarcoma GTPase activating protein) and suppresses Erk (extracellular signal-regulated kinase) signaling, which in turn promotes myogenesis. In lymphoid tissues, it modulates DC homeostasis, which is facilitated by steady state as well as proliferative T-cells.

Sequence

MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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