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SRP3212 Sigma-Aldrich

IL-5 from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: BCDFII, EDF, TRF

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation,
biological source   mouse
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤2.0 ng/mL ED50
mol wt   mol wt 26.2 kDa
packaging   pkg of 25 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   P04401
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... IL5(16191)

Description

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Biochem/physiol Actions

IL-5 (interleukin 5) was initially identified as a stimulant of antibody production by activated B cells. It has recently been shown to be a key factor in eosinophil differentiation and proliferation. It is also implicated in the pathogenesis of asthma, atopy, hypereosinophilic syndromes, and parasitic infections. In distal lung, IL-5 recruits eosinophils and modulates inflammatory or immune cells in the microenvironment, thus, promoting lung metastasis in cancer.

General description

IL-5 (interleukin 5) is a Th2 (T helper 2) cytokine that is predominantly produced by T lymphocytes, and to a lower extent by mast cells, type 2 innate lymphoid (ILC2) cells, and eosinophils.
Recombinant murine IL-5 is a 26.2kDa disulfide-linked homodimeric protein containing two 113 amino acid chains.

Sequence

MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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