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SRP3235 Sigma-Aldrich

SF-20 from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: IL-25

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Description

Biochem/physiol Actions

Murine SF20 is a bone marrow stroma-derived growth factor. Recombinant murine SF20 is a 15.8 kDa protein containing 143 amino acid residues.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized with no additives.

General description

Murine SF20 is a bone marrow stroma-derived growth factor. SF20 is expressed in bone marrow, spleen stroma cells, resting mononuclear cells, resting CD8+ and CD19+ cells and activated CD8+ T cells and has been shown to bind to the surface of cells expressing the receptor TSA-1 (Thymic shared Ag-1). Among its biological activities, SF20 stimulates the proliferation of FDCP2 cells (a mouse factor-dependent hemopoietic cell line) and mouse lymphoid cells. Recombinant murine SF20 is a 15.8 kDa protein containing 143 amino acid residues.

Sequence

MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Request a Serum Sample

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Protocols & Articles

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