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SRP3236 Sigma-Aldrich

TPO from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand

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Properties

Related Categories Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
biological source   mouse
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   <1.0 ng/mL ED50
mol wt   mol wt 18.7 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white
suitability   cell culture tested
UniProt accession no.   P40226
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... THPO(21832)

Description

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of ?0.5 mg/ml. Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 8.0.

Biochem/physiol Actions

TPO (thrombopoietin) is the key regulator of thrombopoiesis for platelet lineage. In both mice and humans, inactivation of this gene results in significant decrease in number of mature platelets produced by marrow progenitors. Transgenic mice with increased expression of this protein show progressive fibrosis and osteosclerosis of the bone marrow, as well as histological changes in kidneys, leading to mesangioproliferative glomerulopathy.

General description

TPO (thrombopoietin) is a hematopoietic cytokine expressed in multiple tissue types. It is predominantly produced by liver and kidney and acts as a ligand for the cell surface receptor c-mpl.

TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant murine TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein.

Sequence

SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


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Protocols & Articles

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Peer-Reviewed Papers
15

References

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