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SRP3247 Sigma-Aldrich

M-CSF from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: CSF-1, MGI-IM, Macrophage Colony Stimulating Factor

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation,
biological source   rat
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤5.0 ng/mL ED50
mol wt   mol wt 18.1 kDa
packaging   pkg of 10 μg
impurities   <0.2 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   Q8JZQ0
shipped in   wet ice
storage temp.   −20°C
Gene Information   rat ... CSF1(78965)

Description

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 7.5.

Biochem/physiol Actions

Macrophage colony stimulating factor (M-CSF) is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation.

General description

Macrophage colony stimulating factor (M-CSF) is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts.The gene encoding this protein is localized on rat chromosome 2q34. The human protein is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant rat M-CSF is a 36.2kDa homodimeric protein containing two 155 amino acid polypeptide subunits.

Sequence

MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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