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SRP3249 Sigma-Aldrich

MIP-1 beta from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: ACT-2, CCL4

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Chemokines, Cytokines and Growth Factors,
biological source   rat
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
mol wt   7.8 kDa
packaging   pkg of 20 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
suitability   cell culture tested
UniProt accession no.   P50230
shipped in   wet ice
storage temp.   −20°C
Gene Information   rat ... CCL4(116637)

Description

General description

Both MIP-1α and MIP-1β are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1α and MIP-1β exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1α selectively attracting CD8+ lymphocytes and MIP-1β selectively attracting CD4+ lymphocytes. Additionally, MIP-1α and MIP-1β have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells. Recombinant rat MIP-1a is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Biochem/physiol Actions

Both MIP-1a and MIP-1b are structurally and functionally related CC chemokines. Recombinant rat MIP-1b is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Sequence

APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles

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Peer-Reviewed Papers
15

References

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