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SRP3255 Sigma-Aldrich

TPO from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand

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Properties

Related Categories Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
biological source   rat
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤0.2 ng/mL ED50
mol wt   18.7 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white
suitability   cell culture tested
UniProt accession no.   P49745
shipped in   wet ice
storage temp.   −20°C
Gene Information   rat ... THPO(81811)

Description

General description

Thrombopoietin (TPO) also known as c-Mpl ligand is an acidic glycoprotein expressed mainly in the liver, kidney, and bone marrow. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7kDa), which contains the erythropoietin-like domain of the full length TPO protein.

Application

Thrombopoietin (TPO) from rat has been used in experimental systems to determine the effect of TPO in recovery from thrombocytopenia.

Biochem/physiol Actions

Thrombopoietin (TPO) acts as a key physiologic regulator of megakaryocyte and platelet production in vivo. Mutation of TPO or its receptor might lead to thrombocytopenia and megakaryocytopoiesis. In addition, TPO also influence the action of erythroid and erythromegakaryocytic progenitors in mouse embryonic yolk sac. TPO binds to c-mpl receptor and regulates circulating platelet level.

Sequence

SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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