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SRP3256 Sigma-Aldrich

TPO from rat

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested

Synonym: MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand

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Properties

Related Categories Bioactives and Supplements for Stem Cell Expansion, Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
biological source   rat
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   ≤0.2 ng/mL ED50
mol wt   mol wt 18.7 kDa
packaging   pkg of 10 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white
suitability   cell culture tested
UniProt accession no.   P49745
shipped in   wet ice
storage temp.   −20°C
Gene Information   rat ... THPO(81811)

Description

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from Sodium Citrate, pH 4.5.

Biochem/physiol Actions

TPO (thrombopoietin) is the key regulator of thrombopoiesis for platelet lineage. In both mice and humans, inactivation of this gene results in significant decrease in number of mature platelets produced by marrow progenitors. Transgenic mice with increased expression of this protein show progressive fibrosis and osteosclerosis of the bone marrow, as well as histological changes in kidneys, leading to mesangioproliferative glomerulopathy.

General description

TPO (thrombopoietin) is a hematopoietic cytokine expressed in multiple tissue types. It is predominantly produced by liver and kidney and acts as a ligand for the cell surface receptor c-mpl.

Sequence

SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles

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Peer-Reviewed Papers
15

References

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