EMAIL THIS PAGE TO A FRIEND
SRP3261 Sigma-Aldrich

FGF-4 human

Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: HBGF-4, HST-1, transforming protein KS3

Purchase

Properties

Related Categories Cell Biology, Cell Culture, Cytokines, Growth Factors, Chemokines for Stem Cell Biology, FGF-4 to FGF-8, Fibroblast Growth Factors (FGF),
biological source   human
recombinant   expressed in E. coli
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
potency   ≥0.5 ng/mL
mol wt   mol wt 19.7 kDa
packaging   pkg of 25 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
UniProt accession no.   P08620
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... FGF4(2249)

Description

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.6 + 100 mM NaCl.

Application

• FGF-4 human has been used to study the effect of FGF4 on the differentiation of PDMCs (placenta-derived multipotent cells) into hepatocyte-like cells.
• It has been used as a growth factor to study the proliferation and differentiation of human bone marrow mesenchymal stem cells (hMSCs) to chondrocytes.
• It has been used to study hepatic differentiation.

Biochem/physiol Actions

FGF4 (fibroblast growth factor 4) was first identified as an oncogene transforming NIH3T3 (mouse embryo fibroblast cell line) cells. It helps in maintaining the self-renewal capability of normal stem cells (for instance, trophoblastic stem cells). It is also required for cellular differentiation, morphogenesis and proliferation. It controls proliferation and differentiation of normal stem cells, progenitor cells and cancer cells.

General description

The FGF4 (fibroblast growth factor 4) gene is mapped to human chromosome 11q13.3. It is expressed during early embryonic development. In adults, it is strongly expressed in the testis. The encoded protein mainly interacts with FGFR1c (FGF receptor 1c) and FGFR2c. It also binds to FGFR3c and FGFR4, but to a lesser extent.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?