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SRP3283 Sigma-Aldrich

FETUIN A human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: Alpha-2-HS-glycoprotein

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Serum Proteins, Serum Proteins and Related Enzymes More...
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
potency   ≥100 nM
mol wt   45.0-55.0 kDa
packaging   pkg of 50 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white to off-white
UniProt accession no.   P02765
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... AHSG(197)

Description

General description

The gene AHSG (α-2-HS-glycoprotein, also referred to as fetuin-A) is mapped to human chromosome 3q27. Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. Fetuin A is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. The protein has cystatin-like domains, a calcium phosphate-binding site and a TGF (transforming growth factor)-β cytokine-binding motif. Recombinant human fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant fetuin-A migrates at an apparent molecular weight of 45–55kDa by SDS-PAGE run under nonreducing conditions.

Application

FETUIN A human has been used to stimulate mRNA expression in human monocytic THP1 cells, human in vitro differentiated adipocytes and C57BL/6 mice.

Biochem/physiol Actions

AHSG (α-2-HS-glycoprotein, also referred to as fetuin-A) is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β (transforming growth factor β) and BMP (bone morphogenetic protein) signaling proteins. High levels of fetuin-A in the serum is associated with mild osteogenesis imperfecta. On the other hand, reduced levels are seen in Paget′s disease. It is predicted as a potential biomarker for the early diagnosis of hypopharyngeal squamous cell carcinoma.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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