SRP3292 Sigma-Aldrich

R-SPONDIN-1 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: Rspo1



Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytoskeleton and Extracellular Matrix, Organelle Proteins and Related Molecules
biological source   human
recombinant   expressed in CHO cells
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
potency   1.0-3.0 μg/mL ED50
mol wt   26.0-40.0 kDa
packaging   pkg of 20 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white
UniProt accession no.   Q2MKA7
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... RSPO1(284654)


General description

R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the (Rspo) family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in adrenal glands, ovary, testis, thyroid, and trachea. Recombinant human R-Spondin-1 is a 26.7kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0kDa by SDS PAGE analysis under reducing conditions.

Biochem/physiol Actions

Rspo (R-Spondin) can interact with the Frizzled/LRP6 (LDL receptor related protein 6) receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Disruption of Rspo-1 gene causes recessive syndrome with XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Rspo-1 is also associated with radioresistance of tumor cells.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.


Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


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Protocols & Articles


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