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SRP3296 Sigma-Aldrich

WNT-7A human

recombinant, expressed in HEK 293 cells, ≥80% (SDS-PAGE), ≥80% (HPLC)

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Properties

Related Categories Adhesion Molecules and Related Proteins, Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥80% (HPLC)
  ≥80% (SDS-PAGE)
form   lyophilized
potency   40-60 ng/mL ED50
mol wt   mol wt 35.5 kDa
packaging   pkg of 15 μg
impurities   <0.1 EU/μg endotoxin, tested
color   white
UniProt accession no.   O00755
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... WNT7A(7476)

Description

Biochem/physiol Actions

Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. Recombinant human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of ≤0.2 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized with no additives.

General description

Wnt-7a is expressed in placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors and induces the expression of numerous genes. Increased Wnt/β-Catenein signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

Wnt/β-Catenin Signaling

Background Wnt Signaling Components    a) Wnt protein      b) Receptors of Wnt proteins    c) Wnt signaling modulators Wnt /beta-catenin signaling mechanism    a) Wnt signaling – Off    b) Wnt signal...
Keywords: Adhesion, Apoptosis, Cancer, Cell division, Cell proliferation, Clinical, Degradations, Gene expression, Hormones, Ligands, Metabolism, Phosphorylations, Transcription, Transduction, Ubiquitinations

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Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
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Peer-Reviewed Papers
15

References

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