EMAIL THIS PAGE TO A FRIEND
SRP3330 Sigma-Aldrich

IL-6 from mouse

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym: BSF-2, HPGF, IFN-?2, B cell differentiation factor (BCDF)

Purchase

Properties

Related Categories CNTF Family - includes ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF) and interleukin-6 (IL-6), Cell Culture, Growth Factors and Cytokines, Interleukin-6, Interleukins (IL),
biological source   mouse
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   5x10^7 units/mg ED50
mol wt   21.7 kDa
packaging   pkg of 10 μg
impurities   endotoxin, tested
color   white to off-white
UniProt accession no.   P08505
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... IL6(16193)

Description

General description

Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant murine IL-6 is a 21.7 kDa protein containing 188 amino acid residues.

Application

IL-6 from mouse has been used to study IL-6-mediated inhibition of mouse preadipocyte differentiation.

Biochem/physiol Actions

IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R and gp130.

Physical form

Lyophilized from 0.1% TFA

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Please Enter a Lot Number

Pipette Controller from Brand

Cell Culture Guide
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?