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SRP4037 Sigma-Aldrich

FGF-2 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: FGF-2, Fibroblast Growth Factor-basic, HBGF-2, Prostatropin

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines, Cytokines and Growth Factors,
biological source   human
recombinant   expressed in E. coli
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
mol wt   mol wt ~17.2 kDa
packaging   pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   endotoxin, tested
NCBI accession no.   NM_002006
UniProt accession no.   P09038
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... FGF2(2247)

Description

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Physical form

Lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 plus 150 mM NaCl.

Application

FGF2 (fibroblast growth factor 2)-human has been used in endothelial colony assay.

Biochem/physiol Actions

FGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3. It stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It plays an important role in spinal cord injury repair. FGF2 is known to stimulate angiogenesis and is associated with cancer development. High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases. The FGF2 mRNA is a target of miRNA miR-503.

General description

The gene FGF2 (fibroblast growth factor 2) is mapped to human chromosome 4q28. FGF-2 is a basic heparin binding growth factor. The recombinant human FGF-2 is a 17.2kDa protein containing 154 amino acid residues. It is also referred to as BFGF (basic fibroblast growth factor).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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