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SRP4244 Sigma-Aldrich

MIP-1alpha human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym: G0S19-1, LD78α, MIP-1-α, chemokine (C-C motif) ligand 3

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Description

General description

MIP-1α (microphage inflammatory protein-1α), or CCL3 (C-C motif chemokine ligand 3), was originally isolated from the supernatant of endotoxin-stimulated murine macrophages. It is an 8kDa chemokine; chemokines are a type of chemotactic cytokines.

Biochem/physiol Actions

MIP-1α (microphage inflammatory protein-1α), or CCL3 (C-C motif chemokine ligand 3), and its receptors- CCR1 and CCR5- promote tumor growth and control osteoclast differentiation, thereby contributing to bone disease progression in multiple myeloma. It suppresses the expression of miR-374b through JNK (Janus kinase), ERK (extracellular signal-regulated kinases), and p38 pathways, thus, facilitating VEGF-A (vascular endothelial growth factor) expression and angiogenesis in human osteosarcoma cells. In chondrosarcoma cells, this protein promotes the up-regulation of matrix metalloproteinase-2 (MMP)-2, thus, promoting cell migration and metastasis.

Physical form

Sterile filtered and then lyophilized without any additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can be diluted into other aqueous buffers.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Peer-Reviewed Papers
15

References

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