SRP4596 Sigma-Aldrich

IFN-alpha 1 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: IFN-α 1, IFNA1, Interferon-α



Related Categories Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors,
biological source   human
recombinant   expressed in E. coli
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
potency   1x10^8 units/mg
mol wt   ~19.3 kDa
packaging   pkg of 100 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   endotoxin, tested
NCBI accession no.   NM_024013
UniProt accession no.   P01562
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... IFNA1(3439)


General description

IFN-α1 (interferon α 1) is a type 1 interferon. Human IFN-a1 is a 19.3kDa protein containing 166 amino acid residues. IFN-α1 belongs to the type I interferon (IFN) family and is present in leukocytes. The IFN-α1 gene is mapped to human chromosome 9p21.3.


IFN-alpha 1 human has been used:
• to treat Vero and breast cancer (MDA-MB-231) cells in interferon assay before viral infection
• as a ligand to vaccinia virus B18R protein in analytical size exclusion chromatography
• to treat chikungunya infected human embryonic kidney cell line (HEK293T) to test its effect on Signal transducer and activator of transcription 1 (STAT1) phosphorylation
• as recombinant interferon to treat lung fibroblast cell line (IMR90) to monitor histone chaperone (HIRA) response

Biochem/physiol Actions

IFN-α1 (interferon α 1) is a lymphoid factor that possesses potent anti-viral activity. IFN-α1 interacts with IFNAR1 (interferon α receptor 1) and IFNAR2. The interaction activates JAK1 (janus-activated kinase 1) and tyrosine kinase 2. These events phosphorylate and activate STAT1 and STAT2 (signal transducer and activator of transcription proteins), leading to the upregulation of interferon-stimulated genes some of which encode antiviral proteins. IFN-alpha 1 (IFN-α1) mediates apoptosis in specific tissues. It may be useful in treating laryngeal carcinoma. The interaction of microRNA, miR-181b with IFN-α1, is regarded as a risk factor in systemic lupus erythematosus.

Physical form

Lyophilized without any additives.


Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles

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