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SRP4675 Sigma-Aldrich

Noggin human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: NOG, SYM1, SYNS1

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Properties

Related Categories BMPs and Receptors, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors,
recombinant   expressed in E. coli
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
mol wt   mol wt ~23.1 kDa
packaging   pkg of 20 μg
impurities   endotoxin, tested
NCBI accession no.   Q13253
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... NOG(9241)

Description

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1 to 1.0 mg/mL. Note: Due to solubility reasons the protein should be kept at low pH. This solution can then be diluted into other aqueous buffers.

Physical form

Lyophilized without any additives.

Application

Noggin (NOG) human has been used as a BMP antagonist.

Biochem/physiol Actions

Noggin (NOG) was originally identified as a BMP-4 (bone morphogenetic protein 4) antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. In mice, loss of noggin causes malformations in skeletal system. In mouse noggin is associated with tissue patterning by participating in cell differentiation, cell proliferation and apoptosis in the developing embryo. Mutations in the gene is associated with several disorders, including proximal symphalangism, multiple synostoses, tarsal/carpal coalition syndrome and Teunissen–Cremers syndrome. It is a gene which is also linked with conductive hearing loss. Polymorphism in the gene might be associated with reduced risk of nonsyndromic cleft lip with or without cleft palate (NSCLP).

General description

Noggin (NOG) belongs to a group of diffusible proteins which bind to ligands of the TGF (transforming growth factor)-β family and regulate their activity by inhibiting their access to signaling receptors. The protein is secreted and exists as a disulfide-linked homodimer. The gene NOG is mapped to human chromosome 17q22. Recombinant human Noggin is a 23.1kDa non-disulfide-linked homodimer consisting of a total of 206 amino acid residues.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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