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SRP4702 Sigma-Aldrich

CTGF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: connective tissue growth factor

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Hormones,
biological source   human
recombinant   expressed in E. coli
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
potency   501.0-2.0 μg/mL ED50
mol wt   ~11.2 kDa
packaging   pkg of 20 μg
impurities   endotoxin, tested
NCBI accession no.   NM_001901
UniProt accession no.   P29279
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CTGF(1490)

Description

General description

The gene CTGF (connective tissue growth factor) is mapped to human chromosome 6q23.2. CTGF is a member of the CCN (Cyr61, CTGF , Nov) family of secreted cysteine rich regulatory proteins and is the major mitogenic and chemoattractant protein produced by umbilical vein and vascular endothelial cells. The protein has an N-terminal secretory signal peptide and four domains (an insulin-like growth factor-binding protein domain, a Von Willebrand factor domain, a thrombospondin type-1 repeat module, and a CT). It is expressed in various tissues, such as connective tissue, heart, brain, kidney, lung, liver, muscle, pancreas gland and placenta. Recombinant human CTGF is a 11.2Da protein of 98 amino acid residues.

Biochem/physiol Actions

CTGF (connective tissue growth factor) stimulates the proliferation and differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, and epithelial cells. It binds to IGF (insulin growth factor), TGF (transforming growth factor) β1 and BMP (bone morphogenetic protein)-4. CTGF plays a crucial role in the progression of diabetic nephropathy. It can work as an oncoprotein in glioma and melanoma. However, it works as a tumor suppressor in lung cancer and colon cancer.

Physical form

Sterile filtered and Lyophilized without additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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