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SRP4754 Sigma-Aldrich

WNT-1 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym: INT-1, WNT1

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Properties

Related Categories Adhesion Molecules and Related Proteins, Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
potency   1.5-2.5 ng/mL ED50
mol wt   mol wt ~38.4 kDa
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   endotoxin, tested
NCBI accession no.   P04628
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... WNT1(7471)

Description

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 μg/μL. The solution can then be diluted into other aqueous buffers.

Physical form

Sterile filtered and lyophilized with no additives.

Application

Wnt family member 1 (Wnt-1) has been used in the differentiation medium for human neural progenitor cells (hNP1).

Biochem/physiol Actions

Wnt family member 1 (Wnt-1) and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over 50 genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers.

General description

Wnt family member 1 (Wnt-1) is a secreted protein that signals through the Frizzled family of cell surface receptors. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. The gene encoding this protein is localized on human chromosome 12q13.12. Recombinant human Wnt-1 is a 38.4kDa, non-glycosylated protein containing 343 amino acid residues.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


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Cell Culture Guide
Protocols & Articles

Articles

Growth Factors in Stem Cell Biology

Growth factors are naturally occurring regulatory molecules, which bind to receptors on the cell surface. They stimulate cell and tissue function through influencing cell differentiation by changing ...
Keywords: Adhesion, Angiogenesis, Cell biology, Cell proliferation, Cellular processes, Diseases, Growth factors, Hormones, Stem cell biology, Therapeutic uses, Transcription, Transfection

Wnt/β-Catenin Signaling

Background Wnt Signaling Components    a) Wnt protein      b) Receptors of Wnt proteins    c) Wnt signaling modulators Wnt /beta-catenin signaling mechanism    a) Wnt signaling – Off    b) Wnt signal...
Keywords: Adhesion, Apoptosis, Cancer, Cell division, Cell proliferation, Clinical, Degradations, Gene expression, Hormones, Ligands, Metabolism, Phosphorylations, Transcription, Transduction, Ubiquitinations

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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