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SRP4817 Sigma-Aldrich

Keratin-8 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: CK-8, Cytokeratin-8, K8, KRT8, Keratin type II cytoskeletal 8, Keratin-8

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
biological source   human
recombinant   expressed in E. coli
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
mol wt   53.5 kDa
packaging   pkg of 5 μg
impurities   endotoxin, tested
NCBI accession no.   P05787
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... K8(3856)

Description

General description

The gene K8 (keratin 8) is mapped to human chromosome 12q13.13. It belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. The protein has an amino-terminal head, a conserved α-helical central rod domain and a carboxyl-terminal tail domain. The recombinant human keratin-8 is expressed in Escherichia coli and purified by proprietary chromatographic techniques.

Biochem/physiol Actions

K8 (keratin 8) is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18. Mutations in KRT8 are associated with liver injury as well as liver diseases. It is also an autoantigen of rheumatoid arthritis.

Physical form

5 μg, sterile-filtered and then lyophilized from 30 mM Tris-HCl, pH 8.0, 9.5 M Urea, 2 mM DTT, 2 mM EDTA, and 10 mM methylammonium chloride

Reconstitution

Reconstitute in water to a concentration of 100ug/mL. This solution can then be diluted into other aqueous buffers.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
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References

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