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SRP4987 Sigma-Aldrich

Otoraplin (OTOR) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym: FDP, Fibrocyte-derived protein, MIAL, Melanoma inhibitory activity-like protein, OTOR, Otoraplin

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytoskeleton and Extracellular Matrix, Extracellular Matrix, Extracellular Matrix Peptides and Proteins More...
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (HPLC)
  ≥98% (SDS-PAGE)
form   lyophilized
mol wt   ~12.7 kDa
packaging   pkg of 20 μg
impurities   endotoxin, tested
NCBI accession no.   Q9NRC9
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... OTOR(56914)

Description

General description

TOR proteins is also known as fibrocyte-derived protein (Fdp) and Melanoma inhibitory activity-like (MIAL). Otoraplin is a member of the melanoma-inhibiting activity gene family. Otoraplin is a secreted 16 kDa globular protein that is expressed in the inner ear by periotic mesenchyme and developing and mature fibrocytes. OTOR is highly homologous to MIA/cartilage-derived retinoic acid-sensitive protein (CD-RAP), which is a cartilage-specific protein that is also expressed in malignant melanoma cells. The 111 amino acid mature human otoraplin contains 1 SH3 domain (46 –107 amino acids) and a Tyr at position 50 that is reportedly sulfated. Otoraplin takes part in the initiation of periotic mesenchyme chondrogenesis. Otoraplin is secreted through the Golgi apparatus and plays a role in cartilage development and maintenance. A frequent polymorphism in the translation start codon of OTOR can abolish translation and may be associated with forms of deafness. Recombinant human Otoraplin produced in E. coli is a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12.7 kDa.The OTOR is purified by proprietary chromatographic techniques.

Physical form

The OTOR protein was lyophilized from a concentrated (1 mg/ml) solution containing 20 mM PBS pH-7.4 and 130 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in sterile dH2O to a concentration of 0.1 -1 mg/mL. This solution can then be diluted into other aqueous buffers.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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