• USA Home
  • SRP5004 - AMPK (α2/β2/γ2), active, His tagged human

EMAIL THIS PAGE TO A FRIEND
SRP5004 Sigma-Aldrich

AMPK (α2/β2/γ2), active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: AAKG2, AAKG, AMPK2, AMPK, CMH6, H91620p, MGC61468, PRKAA2, PRKAA, PRKAB2, PRKAG2, WPWS

Purchase

Properties

Related Categories Active Kinases, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology
recombinant   expressed in baculovirus infected Sf9 cells
grade   PRECISIO® Kinase
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   255-345 nmol/min·mg
mol wt   ~36 kDa (B2)
  ~65 kDa (G2)
  ~69 kDa (A2)
NCBI accession no.   NM_001040633
  NM_005399
  NM_006252
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PRKAA2(5563), PRKAB2(5565), PRKAG2(51422)

Description

General description

AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?