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SRP5006 Sigma-Aldrich

CDC25B, active, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

  •  NACRES NA.32

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Cycle Proteins, Antibodies and Reagents, Cell Cycle Regulators, Cell Signaling and Neuroscience More...
recombinant   expressed in baculovirus infected Sf9 cells
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   18-24 nmol/min·mg
mol wt   ~110 kDa
NCBI accession no.   NM_021873
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... CDC25B(994)

Description

General description

CDC25B (also known as cell division cycle 25 homolog B) is a member of the CDC25 family of phosphatases that activate the cyclin dependent kinase CDC2 by removing two phosphate groups. CDC25B is required for cell entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. Cdc25B is the first genetic model for studying the mechanisms regulating prophase arrest in vertebrates. The regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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