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SRP5149 Sigma-Aldrich

UCHL1, His tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: PARK5, PGP 9.5, PGP9.5, PGP95, Uch-L1

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Intracellular Protein Degradation, Neurobiology, Neuroscience,
biological source   human
recombinant   expressed in E. coli
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
mol wt   ~25 kDa
application(s)   cell analysis: suitable
NCBI accession no.   NM_004181
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... UCHL1(7345)

Description

Biochem/physiol Actions

UCHL1 is a member of the peptidase C12 family and a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to generate the ubiquitin monomer. UCHL1 is expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in UCHL1 may be associated with Parkinson disease. The ligase and hydrolase activities of UCHL1 may play roles in proteasomal protein degradation, a process critical for neuronal health. Inhibition of UCHL1 in mouse hippocampal slices reduces normal synaptic function and long-term potentiation. PKA-CREB pathway mediates the effects of UCHL1 on Amyloid-beta-induced synaptic dysfunction and Uchl1 also reversed the inhibition of CREB phosphorylation induced by Amyloid-beta.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Parkinson's Disease

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease, affecting more than 6 million people worldwide. PD is a slowly progressing motor system neurod...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 16–20
Keywords: Alzheimer Disease, Apoptosis, Chromatin immunoprecipitation, Clinical, Degradations, Dopamine agents, Gene expression, Parkinson Disease, Polymorphisms

Peer-Reviewed Papers
15

References

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