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SRP6057 Sigma-Aldrich

Activin A from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym: FRP, FSH-releasing protein, Inhibin beta-1

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, Reagents and Supplements, Recombinant Activin proteins, Transforming Growth Factors More...
biological source   mouse
recombinant   expressed in E. coli
assay   ≥95% (HPLC)
  ≥95% (SDS-PAGE)
form   lyophilized
mol wt   mol wt 26.2 kDa
packaging   pkg of 10 μg
NCBI accession no.   P08476
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... INHBA(3624)

Description

Other Notes

MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS

Preparation Note

Centrifuge the vial prior to opening.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water, which can then be further diluted to other aqueous solutions.

Physical form

Recombinant mouse Activin A is lyophilized with 0.02% TFA.

Application

Activin A has been used:
• to induce differentiation of embryonic stem cells to pancreatic β cells
• in the induction of bone marrow-derived mesenchymal stem cells to insulin-producing cells
• to induce endoderm differentiation from human embryonic stem cells
• as a component of β cell induction medium.

Biochem/physiol Actions

Activins interact with Type I and Type II serine/threonine kinases to signal to SMAD proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, metabolism, etc. Activin A is a homodimer of two β A chains and is not biologically active until the N terminal propeptide is cleaved from each chain. Activin A is involved in mesoderm formation and hematopoietic development.

General description

Activin A is a member of the TGF (transforming growth factor)-β family of proteins produced by many cell types throughout development. Mouse Activin A has 100% amino acid sequence identity to human, rat, porcine, bovine and feline proteins. Recombinant mouse Activin A is a nonglycosylated homodimer, containing two 117 amino acid chains, with a total molecular weight of 26.2 kDa.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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