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SRP6178 Sigma-Aldrich

TACE, human

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

Synonym: ADAM17, CD156b, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, TNF-alpha convertase

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, T-TB
biological source   human
mol wt   62 kDa
form   lyophilized powder
shipped in   dry ice
storage temp.   −70°C
packaging   pkg of 20 μg
recombinant   expressed in insect cells
assay   ≥90% (SDS-PAGE)
NCBI accession no.   P78536
Gene Information   human ... ADAM17(6868)

Description

General description

A disintegrin and metallopeptidase domain 17 (ADAM17) belongs to ADAM family of genes, which has a characteristic cysteine rich region called disintegrin and a metalloproteinase domain. This gene is located on chromosome 2, spans 55kb and has 19 exons. In adult humans, ADAM17 is expressed in multiple tissues such as, heart, muscle, kidney, placenta, pancreas, thymus, small intestine, ovaries, testes and prostate. In fetus, it is expressed in brain, lung, liver and kidney.

Physical form

The untagged rh-TACE (TNF-alpha converting enzyme) is supplied as a lyophilized powder.

Reconstitution

With sterile deionized water at 0.1 μg protein/μL.

Biochem/physiol Actions

ADAMs (A disintegrin and metallopeptidases) are called ectodomain sheddases and ADAM17 is an angiotensin-converting enzyme 2 (ACE2) sheddase. It also processes and sheds multiple cell surface molecules such as, adhesion molecules and cytokines. It produces the soluble form of tumor necrosis factor α (TNF-α), along with the processing of I-CAM (cell adhesion molecule), V-CAM, IL-6R, L-selectin etc. These in turn play a role in development, regeneration and inflammation of the circulatory system. Polymorphism rs12692386 in ADAM17 gene is associated with an increased susceptibility to abdominal aortic aneurysm. This gene is also up-regulated in myocardial infarction patients, as well as in atherosclerosis patients. It also plays a role in the pathophysiology of ischemic strokes (IS). ADAM17 aids in tumor progression by facilitating tumor proliferation and invasion, as in the case of non-small cell lung cancer (NSCLC).

Preparation Note

Centrifuge the vial prior to opening.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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