SRP6327 Sigma-Aldrich

CD274 from mouse

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

Synonym: A530045L16Rik, B7h1, CD274 antigen, Pdcd1l1, Pdcd1lg1, Pdl1



Related Categories CD160 to CD340, Cell Biology, Cell Signaling and Neuroscience, Cluster of Differentiation (CD) Proteins, Cytokines and Growth Factors,
biological source   mouse
recombinant   expressed in E. coli
assay   ≥85% (SDS-PAGE)
form   liquid
mol wt   27.6 kDa (246 aa, 19-239 aa + His tag)
packaging   pkg of 100 μg
concentration   1 mg/mL
impurities   <1 EU/μg endotoxin (LAL test)
UniProt accession no.   Q9EP73
shipped in   dry ice
storage temp.   −70°C
Gene Information   mouse ... CD274(60533)


General description

Programmed cell death 1 ligand 1 (PD-L1), which is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules. PD-L1 is a cell surface immunoglobulin with two Ig-like domains within the extracellular region and a short cytoplasmic domain. The protein is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. Recombinant mouse CD274 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Biochem/physiol Actions

Programmed cell death 1 ligand 1 (PD-L1) is involved in the regulation of cellular and humoral immune responses. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after lipopolysaccharide (LPS) and interferon-γ (IFNG) activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 is involved in the co-stimulatory signal, essential for T-cell proliferation and production of interleukin-10 (IL10) and IFNG, in an interleukin-2-dependent and a programmed cell death 1 (PDCD1)-independent manner.

Physical form

1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl and 10% glycerol.

Safety & Documentation

Safety Information

NONH for all modes of transport


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